Reduction in the adherence of Pseudomonas aeruginosa to human buccal epithelial cells with neuraminidase inhibition.

The aim of this study was to evaluate the reduction in the adherence of 33 strains of Pseudomonas aeruginosa isolated from humans and different animals to human buccal epithelial cells with neuraminidase inhibition. Buccal epithelial cells were incubated with strains of Pseudomonas aeruginosa in the presence or absence of the neuraminidase inhibitors, 2,3-dehydro-2-deoxy-N-acetyl-neuraminic acid (DANA) or N-acetyl-neuraminic acid (NANA). Incubation of cells with bacteria in the presence of either DANA or NANA reduced bacterial adherence significantly by 35.24 +/- 23.90%, and 68.00 +/- 22.51 %, respectively. We suggest that the in vivo effects of such interventions should be explored as potential mechanisms reducing Pseudomonas aeruginosa in the binding to buccal cells.